ScholarLens Back to search
2010•Anhui nongye kexueRequires access

Determination of Content of Baicalin in Liyan Jiedu Keli by HPLC

Qiang Chen

Open publisher page 0 citations

Abstract

[Objective]To establish a HPLC method for the quantitative determination of Baicalin in Liyan Jiedu Keli.[Methods]The HPLC Conditions were as follows:Agilent zorbax SB-C18 column(4.6 mm×150.0 mm,5 μm),mobile phase of methanol-water-phosphoric acid(V∶V∶V=47∶53∶0.2),flow rate of 1.0 ml/min.detection wavelength of 280 nm and column temperature of 30 ℃ [Results]The better linear range of baicalin was 0.5-100.0 μg/ml(r=0.999 1),the mean recovery was 101.14 % with RSD 0.69 %(n=9).[Conclusion]The method is fast,simple and accurate,which can be applied for the quality control of Liyan Jiedu Keli.

About this research paper

What this paper is about

[Objective]To establish a HPLC method for the quantitative determination of Baicalin in Liyan Jiedu Keli.[Methods]The HPLC Conditions were as follows:Agilent zorbax SB-C18 column(4.6 mm×150.0 mm,5 μm),mobile phase of methanol-water-phosphoric acid(V∶V∶V=47∶53∶0.2),flow rate of 1.0 ml/min.detection wavelength of 280 nm and column temperature of 30 ℃ [Results]The better linear range of baicalin was 0.5-100.0 μg/ml(r=0.999 1),the mean recovery was 101.14 % with RSD 0.69 %(n=9).[Conclusion]The method is fast,simple and accurate,which can be applied for the quality control of Liyan Jiedu Keli.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

[Objective]To establish a HPLC method for the quantitative determination of Baicalin in Liyan Jiedu Keli.[Methods]The HPLC Conditions were as follows:Agilent zorbax SB-C18 column(4.6 mm×150.0 mm,5 μm),mobile phase of methanol-water-phosphoric acid(V∶V∶V=47∶53∶0.2),flow rate of 1.0 ml/min.detection wavelength of 280 nm and column temperature of 30 ℃ [Results]The better linear range of baicalin was 0.5-100.0 μg/ml(r=0.999 1),the mean recovery was 101.14 % with RSD 0.69 %(n=9).[Conclusion]The method is fast,simple and accurate,which can be applied for the quality control of Liyan Jiedu Keli.

Key concepts: Baicalin, High-performance liquid chromatography, Chromatography, Chemistry, Phosphoric acid, Content determination, Linear range, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Content of Baicalin in Liyan Jiedu Keli by HPLC — Research Paper | ScholarLens