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2013•Zhongguo shiyan fangjixue zazhiRequires access

Simultaneous Determination of Baicalin and Emodin in Liyan Jiedu Granules by HPLC

Bai Zi-jing

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Abstract

Objective:To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules,response surface methodology(RSM) was applied to predict optimum conditions for extraction.Method:The analysis was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile(A) and 0.2% phosphoric acid(B) with gradient elution at a flow rate of 1.0 mL · min-1.The detection wavelength were set at 280,245 nm,respectively.The column temperature was 28 ℃.Result:The linear ranges of baicalin and emodin were 2.016-134.4 and 0.408 0-27.20 mg · L-1,respectively.The average recoveries(n=6) of these were 98.9% and 97.8%,respectively.Conclusion:This method is simple,accurate and repeatable,and can be used for the quality control of Liyan Jiedu granules.

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What this paper is about

Objective:To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules,response surface methodology(RSM) was applied to predict optimum conditions for extraction.Method:The analysis was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile(A) and 0.2% phosphoric acid(B) with gradient elution at a flow rate of 1.0 mL · min-1.The detection wavelength were set at 280,245 nm,respectively.The column temperature was 28 ℃.Result:The linear ranges of baicalin and emodin were 2.016-134.4 and 0.408 0-27.20 mg · L-1,respectively.The average recoveries(n=6) of these were 98.9% and 97.8%,respectively.Conclusion:This method is simple,accurate and repeatable,and can be used for the quality control of Liyan Jiedu granules.

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Available abstract

Objective:To develop an HPLC method for the determination of baicalin and emodin in Liyan Jiedu granules,response surface methodology(RSM) was applied to predict optimum conditions for extraction.Method:The analysis was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of acetonitrile(A) and 0.2% phosphoric acid(B) with gradient elution at a flow rate of 1.0 mL · min-1.The detection wavelength were set at 280,245 nm,respectively.The column temperature was 28 ℃.Result:The linear ranges of baicalin and emodin were 2.016-134.4 and 0.408 0-27.20 mg · L-1,respectively.The average recoveries(n=6) of these were 98.9% and 97.8%,respectively.Conclusion:This method is simple,accurate and repeatable,and can be used for the quality control of Liyan Jiedu granules.

Key concepts: Baicalin, Emodin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Extraction (chemistry), Response surface methodology

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