Improvement of the Extraction Method of the Genome DNA from Arthrobacter

[Objective] The different extraction methods of Arthrobacter DNA were compared to obtain high-quality genomic DNA.[Method] The cells were pretreated in repeated freeze-grinding and toluene;then the DNA was extracted with CTAB ion,and finally,the result was compared with the bacterial genomic comparison kit.[Results] The result of the agarose gel electrophoresis of the purified DNA extracted with modified CTAB showed that the genomic DNA bands were clear,homogeneity in measure and same size of DNA fragment.The DNA as a template was amplified by PCR,it had single DNA band and specific trait,in which,the extraction method of DNA pretreated by toluene was rapid,simple,economical and practical,which could be used as a template for PCR amplification and for the follow-up genetic manipulation and molecular biology research.[Conclusion] The modified CTAB method was effectively in the extraction of Arthrobacter genome DNA,which purity was required for follow-up research.