Screening on active principle of Bidens bipinnata L on acute liver injury induced by carbon tetrachloride in mice

Objective To select the active principle extracted from Bidens bipinnata L that can protect aganist acute liver injury,and to explore the hepatoprotective effect.Methods Seventy male Kunming mice were randomly divided into the normal control group,the CCl4 injury model group,the A composition of Bidens bipinnata L group(360 mg/kg),the B composition of Bidens bipinnata L group(180 mg/kg),the C composition of Bidens bipinnata L group(100 mg/kg,and the Bifendate(100 mg/kg) group.All the mice except for those in the normal control group had acute liver injury induced by carbon tetrachloride(CCl4) 10 ml/kg injected intraperitoneally.The animals were killed 18 h after CCl4 administration.The liver index,activities of serum aspartate transaminase(AST),alanine transaminase(ALT),the levels of malondialdehyde(MDA),superoxide dismutase(SOD) in liver homogenate,as well as the pathological changes of the liver were determined in all groups.Results In comparison to the normal control group,the liver index,the level of serum ALT,AST and the content of hepatic MDA were increased significantly in CCl4 injury model group(P0.01),while the activities of hepatic SOD were significantly decreased(P0.01).And the liver histology showed necrosis and degeneration,the disappearance of hepatic lobules and hepatocyte,and the degree of liver pathology lesions increased significantly compared to the normal group(P 0.01).The liver index and serum ALT in groups treated by Bidens bipinnatal were significantly lower than those in model group(P0.01).The liver necrosis and degeneration induced by CCl4 were also improved significantly by Bidens bipinnatal.Conclusion The flavonoids of Bidens bipinnata L have protective effects aganist acute liver injury induced by CCl4 in mice,which maybe the major active principle of hepatoprotective effect of Bidens bipinnata L.