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Effect of small-molecule c-Myc inhibitor 10058-F4 on proliferation and apoptosis of renal cell carcinoma 786-0 cells

Qi Liu

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Abstract

Objective To investigate the effect of small-molecule c-Myc inhibitor,10058-F4,on proliferation and apoptosis of renal cell carcinoma 786-0 cells.Methods Renal cell carcinoma 786-0 cells proliferation and its relationship with concentration and tion were detected by MTT assay,the effects of 10058-F4 on cell cycle and cell apoptosis were determined by FCM,the expression of c-Myc,p21,p27 and Bcl-2 were analyzed by Western blot.Results The proliferation of renal cell carcinoma 786-0 cells were significantly inhibited by 10058-F4 in a concentration-dependent and time-dependent,and 10058-F4 arested cell cycle at G0/G1 phase and induced cell apoptosis,and deregulated the expression of c-Myc and its target gene p21,p27 and upregulated the expression of Bcl-2.Conclusion Small-molecule c-Myc inhibitor,10058-F4,can inhibit the proliferation and induce apoptosi of renal cell carcinoma 786-0 cells in a dose and time-dependent.

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Objective To investigate the effect of small-molecule c-Myc inhibitor,10058-F4,on proliferation and apoptosis of renal cell carcinoma 786-0 cells.Methods Renal cell carcinoma 786-0 cells proliferation and its relationship with concentration and tion were detected by MTT assay,the effects of 10058-F4 on cell cycle and cell apoptosis were determined by FCM,the expression of c-Myc,p21,p27 and Bcl-2 were analyzed by Western blot.Results The proliferation of renal cell carcinoma 786-0 cells were significantly inhibited by 10058-F4 in a concentration-dependent and time-dependent,and 10058-F4 arested cell cycle at G0/G1 phase and induced cell apoptosis,and deregulated the expression of c-Myc and its target gene p21,p27 and upregulated the expression of Bcl-2.Conclusion Small-molecule c-Myc inhibitor,10058-F4,can inhibit the proliferation and induce apoptosi of renal cell carcinoma 786-0 cells in a dose and time-dependent.

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Available abstract

Objective To investigate the effect of small-molecule c-Myc inhibitor,10058-F4,on proliferation and apoptosis of renal cell carcinoma 786-0 cells.Methods Renal cell carcinoma 786-0 cells proliferation and its relationship with concentration and tion were detected by MTT assay,the effects of 10058-F4 on cell cycle and cell apoptosis were determined by FCM,the expression of c-Myc,p21,p27 and Bcl-2 were analyzed by Western blot.Results The proliferation of renal cell carcinoma 786-0 cells were significantly inhibited by 10058-F4 in a concentration-dependent and time-dependent,and 10058-F4 arested cell cycle at G0/G1 phase and induced cell apoptosis,and deregulated the expression of c-Myc and its target gene p21,p27 and upregulated the expression of Bcl-2.Conclusion Small-molecule c-Myc inhibitor,10058-F4,can inhibit the proliferation and induce apoptosi of renal cell carcinoma 786-0 cells in a dose and time-dependent.

Key concepts: Apoptosis, Cell growth, Cell cycle, Renal cell carcinoma, Western blot, Cancer research, MTT assay, Cell

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Effect of small-molecule c-Myc inhibitor 10058-F4 on proliferation and apoptosis of renal cell carcinoma 786-0 cells — Research Paper | ScholarLens