The effection of arsenic trioxide in human lung adenocarcinoma A549 cell line proliferation and c - Myc gene expression

Objective To observe the effects of arsenic trioxide(As2O3) on human lung adenocarcinoma cell line A549 cell growth,proliferation,cell cycle arrest,apoptosis and other aspects,and to explore its mechanism.Methods Choose different concentrations of As2O3 treatment of man lung cancer A549 cell lines.Investigate the inhibition rate of cell growth and proliferation with MTT colorimetric assay.Using flow cytometry to determine the cell cycle and apoptosis index.Using immunohistochemistry method to analysis the influence of different concentrations of As2O3 on the c-Myc gene expression.Results As2O3 inhibited the proliferation of human lung adenocarcinoma A549 cells in a time and concentration dependent manner and induced cell apoptosis.Different concentrations As2O3 treatment group could regulate the cell cycle,cause G2 / M phase arrest and S phase arrest,and negatively regulatethe c-Myc gene expression.Conclusion Arsenic trioxide has a significant inhibited effect on the growth and proliferation of in vitro growing human lung cancer A549.The inhibition in vitro is related to arrest of cell cycle phases,suppression of c-Myc gene expression and apoptosis.