A Study on Inhibitory Effect of Isorhmnetin on Human Lung Cancer Cell

Objective To study the inhibitory effect of isorhmnetin on human lung tumor YTLMC-90 cells and to elucidate its molecular mechanisms. Method Inhibition growth of YTLMC-90 cells was measured by growth curve , clone formation test,3H-thymidine uptake assay ; cell cycle and the expression of genes were analyzed by flow cytometry. Result Growth of YTLMC-90 cells was inhibited evidently after treatment by isorhmnetin and was induced apoptosis. Furthermore, it decreased the expression of bcl-2, c-myc, H-ras oncogene and increased the expression of bax and c-fos. Conclusion Isorhmnetin inhibited cell proliferation and induced apoptosis of YTLMC-90 cells, which might be associated with down-regulation of the expression of bcl-2, c-myc and H-ras oncogenes and up-regulation of expression of bax and c-fos genes.