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Determination Contents of Total flavonoids and Quercetin of Polugomum Perfoliatum L.

Kefeng Zhang

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Abstract

Objective:To establish new methods to determinate the total flavonoids and quercetin of the Polugomum perfoliatum L.Methods:Total flavonoids were determined by UV-Vis spectrophotometry and quercetin by RP-HPLC.Results:Indicated the standard curve of total flavonoids was linear in the range of 0.00864～0.06912mgomL-1(r=0.9998) and its average recovery ratio was 98.6%;The standard curve of the quercetin was linear in the range of 0.008～0.04mgomL-1(r=0.9998)and the average recovery ratio was 98.5%,RSD=1.31%.Conclusion:the method is simple and reliable,can be used to control the quality of Polugomum perfoliatum L.

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What this paper is about

Objective:To establish new methods to determinate the total flavonoids and quercetin of the Polugomum perfoliatum L.Methods:Total flavonoids were determined by UV-Vis spectrophotometry and quercetin by RP-HPLC.Results:Indicated the standard curve of total flavonoids was linear in the range of 0.00864～0.06912mgomL-1(r=0.9998) and its average recovery ratio was 98.6%;The standard curve of the quercetin was linear in the range of 0.008～0.04mgomL-1(r=0.9998)and the average recovery ratio was 98.5%,RSD=1.31%.Conclusion:the method is simple and reliable,can be used to control the quality of Polugomum perfoliatum L.

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Available abstract

Objective:To establish new methods to determinate the total flavonoids and quercetin of the Polugomum perfoliatum L.Methods:Total flavonoids were determined by UV-Vis spectrophotometry and quercetin by RP-HPLC.Results:Indicated the standard curve of total flavonoids was linear in the range of 0.00864～0.06912mgomL-1(r=0.9998) and its average recovery ratio was 98.6%;The standard curve of the quercetin was linear in the range of 0.008～0.04mgomL-1(r=0.9998)and the average recovery ratio was 98.5%,RSD=1.31%.Conclusion:the method is simple and reliable,can be used to control the quality of Polugomum perfoliatum L.

Key concepts: Quercetin, Medicine, Linear relationship, Traditional medicine, Chromatography, Food science, Quality standard, Biochemistry

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Determination Contents of Total flavonoids and Quercetin of Polugomum Perfoliatum L. — Research Paper | ScholarLens