2010•Journal of Inner Mongolia UniversityRequires access

Comparision of Contents of Quercetin and Total Flavonoids of Xanthoceres sorbifolia Bunge from Different Origins

Wanfu Bai

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Abstract

High performance liquid chromatography(HPLC) was employed for determination of quercetin and UV spectrophotometry was used for determination of total flavonoids of Xanthoceres sorbifolia Bunge from different origins.The linear range of quercetin was 0.40μg·mL-1-8.0μg·mL-1(r=0.9992),the average recovery was 99.00%,RSD was 2.44%(n=6).The contents of quercetin in Xanthoceres sorbifolia Bunge from seven different places were in the range of 0.0088%-0.0143%.The linear range of Rutin was 8μg·mL-1-48μg·mL-1(r=0.9993),the average recovery was 101.3%,RSD was 1.45%(n=6).The contents of total flavonoid from seven different places were in the range of 3.27%-10.57%.The highest content of quercetin and total flavonoid occurred in Ordos of Inner Mongolia,the lowest content occurred in Haozhou of Anhui.The method for quantitation of quercetin and total flavonoids of Xanthoceres sorbifolia Bunge was accurate and reliable.It can be used to evaluate the quality of Xanthoceres sorbifolia Bunge.

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High performance liquid chromatography(HPLC) was employed for determination of quercetin and UV spectrophotometry was used for determination of total flavonoids of Xanthoceres sorbifolia Bunge from different origins.The linear range of quercetin was 0.40μg·mL-1-8.0μg·mL-1(r=0.9992),the average recovery was 99.00%,RSD was 2.44%(n=6).The contents of quercetin in Xanthoceres sorbifolia Bunge from seven different places were in the range of 0.0088%-0.0143%.The linear range of Rutin was 8μg·mL-1-48μg·mL-1(r=0.9993),the average recovery was 101.3%,RSD was 1.45%(n=6).The contents of total flavonoid from seven different places were in the range of 3.27%-10.57%.The highest content of quercetin and total flavonoid occurred in Ordos of Inner Mongolia,the lowest content occurred in Haozhou of Anhui.The method for quantitation of quercetin and total flavonoids of Xanthoceres sorbifolia Bunge was accurate and reliable.It can be used to evaluate the quality of Xanthoceres sorbifolia Bunge.

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Available abstract

High performance liquid chromatography(HPLC) was employed for determination of quercetin and UV spectrophotometry was used for determination of total flavonoids of Xanthoceres sorbifolia Bunge from different origins.The linear range of quercetin was 0.40μg·mL-1-8.0μg·mL-1(r=0.9992),the average recovery was 99.00%,RSD was 2.44%(n=6).The contents of quercetin in Xanthoceres sorbifolia Bunge from seven different places were in the range of 0.0088%-0.0143%.The linear range of Rutin was 8μg·mL-1-48μg·mL-1(r=0.9993),the average recovery was 101.3%,RSD was 1.45%(n=6).The contents of total flavonoid from seven different places were in the range of 3.27%-10.57%.The highest content of quercetin and total flavonoid occurred in Ordos of Inner Mongolia,the lowest content occurred in Haozhou of Anhui.The method for quantitation of quercetin and total flavonoids of Xanthoceres sorbifolia Bunge was accurate and reliable.It can be used to evaluate the quality of Xanthoceres sorbifolia Bunge.

Key concepts: Quercetin, Rutin, Chemistry, Flavonoid, Chromatography, High-performance liquid chromatography, Traditional medicine, Linear relationship

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