Identification Seed Purity of Six Maize Hybrids by SSR Markers

Purity of hybrids seed is one of the key factor influencing maize industry.To establish rapid and accurate purity detection technique is an effective measure for maize seed purity controlling.Six primers were selected from 30 pairs of SSR,which gave stable and polymorphic amplification profiles.The amplification bands of hybrids were complementary with that of the parents.Umc 1002,bnlg 1112,bnlg 238,Umc 1153,phi 072 and bnlg 240 could detect the purity of Nongda No.108,Xundan No.20,Zhengdan No.958,Lainong No.14,Lainong No.15 and Luyu No.16,respectively.Alleles frequency of the complement bands were relatively low with a range of 0.028-0.072,which ensured to separate self-cross accurately and off-type individuals with hybrid plants.The results obtained by SSR markers were a little less than that of field plot planting testing,but there were no significant difference when statistical analysis was performed.The correlation coefficient was 0.8434.The SSR identification system established in this paper could be used to identify seed purity of six maize hybrid varieties rapidly.