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Resistance Genes of Multi-drug Resistance Strains of Neisseria gonorrhoeae

Yi‐Ming Fan

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Abstract

OBJECTIVE To investigate the r el ationship between the multi-drug resistance and the resistance genes of Neisseria gonorrhoeae. METHODS Thirty five strains of N. gonorrhoeae were tested their susceptibilities to antibiotics by K-B an d agar dilution methods.Total DNA was extracted by boiling methods.The resistanc e genes of TEM,tetM,erm and mefA were amplified by PCR. RESULTS The susceptibilities tests showed that the multi-drug resistance of N.gonorrhoeae was obvious.Th e percentages of TEM and tetM were 88.6% and 11.4%,respectively.Among the 35 strains,two strains mefA genes were positive,and one strain erm was positive.Th is is the first time to find the resistance genes of erm and mefA from N.gonorrhoeae in our country. CONCLUSIONS It has a close relationship between the multi-drug resistance and the resistance genes of N.gonorrh oeae.

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OBJECTIVE To investigate the r el ationship between the multi-drug resistance and the resistance genes of Neisseria gonorrhoeae. METHODS Thirty five strains of N. gonorrhoeae were tested their susceptibilities to antibiotics by K-B an d agar dilution methods.Total DNA was extracted by boiling methods.The resistanc e genes of TEM,tetM,erm and mefA were amplified by PCR. RESULTS The susceptibilities tests showed that the multi-drug resistance of N.gonorrhoeae was obvious.Th e percentages of TEM and tetM were 88.6% and 11.4%,respectively.Among the 35 strains,two strains mefA genes were positive,and one strain erm was positive.Th is is the first time to find the resistance genes of erm and mefA from N.gonorrhoeae in our country. CONCLUSIONS It has a close relationship between the multi-drug resistance and the resistance genes of N.gonorrh oeae.

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Available abstract

OBJECTIVE To investigate the r el ationship between the multi-drug resistance and the resistance genes of Neisseria gonorrhoeae. METHODS Thirty five strains of N. gonorrhoeae were tested their susceptibilities to antibiotics by K-B an d agar dilution methods.Total DNA was extracted by boiling methods.The resistanc e genes of TEM,tetM,erm and mefA were amplified by PCR. RESULTS The susceptibilities tests showed that the multi-drug resistance of N.gonorrhoeae was obvious.Th e percentages of TEM and tetM were 88.6% and 11.4%,respectively.Among the 35 strains,two strains mefA genes were positive,and one strain erm was positive.Th is is the first time to find the resistance genes of erm and mefA from N.gonorrhoeae in our country. CONCLUSIONS It has a close relationship between the multi-drug resistance and the resistance genes of N.gonorrh oeae.

Key concepts: Neisseria gonorrhoeae, Microbiology, Gene, Drug resistance, Agar Dilution Method, Agar dilution, Biology, Antibiotics

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