Experimental study on synergy and attenuation of Dangguibuxue Decoction to 5-fluorouridine

Objective To observe the synergy and attenuation of Dangguibuxue Decoction to 5-fluorouridine(5-Fu) in treating mice with H22 hepatoma.Methods The well growing ascites was taken out with asepsis to be the source of carcinoma and was diluted with normal saline at 1:3,then was inoculated hypodermically at the right axilla of mouse.24 hours after the inoculation,mice were randomly divided into 5 groups,bearing cancer control group,chemotherapy group,positive control group(Zhenqifuzheng Capsules),high-dose and low-dose of Dangguibuxue Decoction group.After 10 days of treatment,we calculated the tumor suppressing rate,counted the peripheral blood cells and bone marrow karyocytes,took the weight of the spleen and thymus,detected the contents of malondialdehyde(MDA) in small intestine and nitric oxide(NO) in spleen.Results Dangguibuxue Decoction could elevate the tumor suppressing rate of 5-Fu on H22 hepatoma mice(P﹤0.01),antagonize the toxic and side effects of 5-Fu on the atrophy of the spleen and thoracic gland,the decrease of white blood cells,platelets and bone marrow karyocytes.Also it could decrease MDA in small intestine and NO in spleen of H22 hepatoma mice who were treated with 5-Fu(P0.05～0.01).Conclusion Dangguibuxue Decoction has the synergy and attenuation to 5-Fu in treating mice with H22 hepatoma.