Induction and state regulation of callus of Acanthopanax senticosus callus

Objective To induce callus from A.senticosus for cell suspension culture.Methods Using tissue and cell culture technology,the effects on the induction of callus of three hormone combination of dedifferentiation medium and the state of A.senticosus callus of nine different hormone combinations in cell culture were studied.Results The result showed that the callus induced under the condition of α-Naphthaleneacetic acid(NAA)2.0 mg·L-1+N-(phenylmethyl)-1H-purin-6-amine(6-BA)2.0 mg·L-1 was most suitable,the callus induction ratio of the leaf and petiole were 93.3% and 100%,respectively.And the callus grew better under the condition of NAA 1.0 mg·L-1+6-BA 1.0 mg·L-1.Conclusions The suitable callus for the suspension culture is obtained by using spire and petiole of A.senticosus as explants with NAA 2.0 mg·L-1+6-BA 2.0 mg·L-1 as callus induction media,NAA 1.0 mg·L-1+6-BA 1.0 mg·L-1 as culture media.