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2006•Anhui nongye kexueRequires access

Study on the Condition of Callus Induction of Rehmannia glutinosa Libosch

Menglin Liu

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Abstract

To gain the optimization conditions of the callus induction,the research on the inducement conditions of the callus from the different tobacco explants was conducted and the different culture media were added with various concentrations of 2.4-D,NAA and 6-BA.The result indicated that the rate of callus induction from leaf,petiole and stem segment was 100% on the medium with MS + 1.0～1.5 mg/L NAA,but more callus from leaf was inducted.Two kinds of auxins could induce callus,but the callus grew faster in the medium added with 0.5mg/L 6BA.MS and LS medium was more suitable for the callus induction and the callus from B5 medium grew best.

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What this paper is about

To gain the optimization conditions of the callus induction,the research on the inducement conditions of the callus from the different tobacco explants was conducted and the different culture media were added with various concentrations of 2.4-D,NAA and 6-BA.The result indicated that the rate of callus induction from leaf,petiole and stem segment was 100% on the medium with MS + 1.0～1.5 mg/L NAA,but more callus from leaf was inducted.Two kinds of auxins could induce callus,but the callus grew faster in the medium added with 0.5mg/L 6BA.MS and LS medium was more suitable for the callus induction and the callus from B5 medium grew best.

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Available abstract

To gain the optimization conditions of the callus induction,the research on the inducement conditions of the callus from the different tobacco explants was conducted and the different culture media were added with various concentrations of 2.4-D,NAA and 6-BA.The result indicated that the rate of callus induction from leaf,petiole and stem segment was 100% on the medium with MS + 1.0～1.5 mg/L NAA,but more callus from leaf was inducted.Two kinds of auxins could induce callus,but the callus grew faster in the medium added with 0.5mg/L 6BA.MS and LS medium was more suitable for the callus induction and the callus from B5 medium grew best.

Key concepts: Callus, Petiole (insect anatomy), Explant culture, Rehmannia glutinosa, Botany, Horticulture, Biology, Chemistry

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