The Extraction Technique of Baicalin and Its Content Determination by HPLC

Objective To establish an appropriate extracting technology for Scutellariae Radix extracts by water decoction extraction method,and establish a method for baicalin content determination.Methods The total amount of baicalin in Scutellariae Radix extracts was used as the index by orthogonal design to optimize the best technique.HPLC was adopted for the determination of baicalin in Scutellariae Radix extracts.Results The optimized parameter resulted when the water consumption was 12 times the weight of Scutellariae Radix,which was decocted three times,once per hour.A linearity was obtained from the range of 0.046 52～0.465 2μg with a good correlation coefficient(r=0.999 9,n=6).The average recovery was 99.98%,RSD =1.56%(n=9).Conclusion The extraction technique was appropriate for Scutellariae Radix extracts;the technique was stable,rational and feasible.The HPLC method was accurate,precise,and repeatable in estimating the contents of baicalin in Scutellariae Radix extracts.