Comparison of the Different Methods to Extract Yellow Pigment from Gardenia jasminoides Ellis

The effects of extracting yellow pigment from Gardenia jasminoides Ellis by hot extraction,ultrasonic extraction and microwave-assisted extraction were compared.Orthogonal tests were used to optimize the conditions.Through orthogonal test of heat extraction,the optimum conditions were determined as follows:70 % EtOH as extracting solvent,2 h,30 ℃,1∶25 solid-liquid ratio,the color value of the extracted pigment achieved 66.45.Through orthogonal test of ultrasonic extraction,the optimum conditions were determined as follows: 80 % EtOH as extracting solvent,20 min at 20 ℃,and solid-liquid ratio 1∶20,the color value of the extracted pigment achieved 87.77.Through single factor test of microwave-assisted extraction,the optimum conditions were determined as follows: 800 W,50 % EtOH as extracting solvent,30 min at 50 ℃,and solid-liquid ratio 1∶5,the color value of the extracted pigment achieved 64.14.The results showed that the effects of extraction while by three different methods were different,ultrasonic extraction was the best way.