Preparation and Application of Immunoaffinity Column for Ractopamine,Salbutamol and Clenbuterol

The anti-ractopamine monoclonal antibody and anti-clenbuterol monoclonal antibody were both coupled with CNBr activated Sepharose 4B to make the immunoaffinity column. The column performance was tested and the use conditions were optimized by HPLC-MS /MS. The results showed that the column capacity to ractopamine,salbutamol and clenbuterol were 49,27,24 ng /mL glue,respectively,the storage time was five months,and the best eluent was methanol. The average recoveries of three lean meat essences ranged from 78. 9% to 96. 5% with relative standard deviations( RSD) lower than 1. 5%. The limits of detection( LOD) ranged from 0. 1 ng/g to 1 ng/g. The method could be applied in the simultaneous determination of the lean meat essence contents in feed samples.