Method to Simultaneously Detect Sabutamol,Ractopamine and Clenbuterol in the Meat by High Performance Liquid Chromatography

Objective Establishing a method with high performance liquid chromatography to simultaneously detect sabutamol,ractopamine and clenbuterol in the meat.Methods Samples were extracted with 60%ethanol,pH value above 7,purified by WCX SPE Cartridge,separated on C18 column(diamond) eluted with acetonitrile and 0.01mol/L sodium dihydrogen phosphate of pH value of 3 and retained by DAD detector.Results Good correlation and coefficient of the standard curve were obtained(r0.999) and the recoveries of sample were 70.8%~81.7% with relative standard deviations less than 5%.Conclusion This method is simple,reliable and accurate for determination of sabutamol,ractopamine and clenbuterol in the meat.