The Detection of three β-Excitant Residue in Meats by GC/MS

A method was developed for the simultaneous determination of clenbuterol,salbutamol and ractopamine in meat by gas chromatography -mass spectrometry(GC/MS).The sample was extracted in acetate buffer of pH5.2 and the extraction was hydrolyzed by β-glucuronidase.The supernatant was passed through a SCX cation-exchange column and derivatized with N,O-bis(trimethylsiyl)trifluoroacetamide(BSTFA).The selected ion monitoring(SIM) method was used for detection,and the monitored ions were m/z86,262,243 for clenbuterol,m/z86,369,440 for salbutamol,m/z250,267,502 for ractopamine,respectively.Quantiation ions were selected at m/z86 for clenbuterol,m/z369 for salbutamol,m/z250 for ractopamine.The limits of detection were 0.5ug/kg for Clenbuterol and Salbutamol, 2ug/kg for ractopamine.The correfficients were better than 0.99 for the calibration curves for the drugs.The recoveries were 75%-98%,with relative standard deviations of 5%-14%.