ScholarLens Back to search
2009•Qilu Pharmaceutical AffairsRequires access

Determination the assay of tryptophan in fermentation broth by Fe(AC)_3

Zhou Jing

Open publisher page 0 citations

Abstract

OBJECTIVE To establish a method for tryptophan determination in fermentation broth by Fe(AC)3.METHODS There was a maximum absorption at wavelength of 276nm for the oxide of tryptophan by FeCl3in surfuric acid solution.RESULTS The average recovery was 100.1%,RSD was 0.43%(n=9),the standard curve was linear with a tryptophan range of 300~800μg·mL-1.CONCLUSION This method was quite repeatable and flexible.It can be used for tryptophan determination in fermentation broth.

About this research paper

What this paper is about

OBJECTIVE To establish a method for tryptophan determination in fermentation broth by Fe(AC)3.METHODS There was a maximum absorption at wavelength of 276nm for the oxide of tryptophan by FeCl3in surfuric acid solution.RESULTS The average recovery was 100.1%,RSD was 0.43%(n=9),the standard curve was linear with a tryptophan range of 300~800μg·mL-1.CONCLUSION This method was quite repeatable and flexible.It can be used for tryptophan determination in fermentation broth.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To establish a method for tryptophan determination in fermentation broth by Fe(AC)3.METHODS There was a maximum absorption at wavelength of 276nm for the oxide of tryptophan by FeCl3in surfuric acid solution.RESULTS The average recovery was 100.1%,RSD was 0.43%(n=9),the standard curve was linear with a tryptophan range of 300~800μg·mL-1.CONCLUSION This method was quite repeatable and flexible.It can be used for tryptophan determination in fermentation broth.

Key concepts: Tryptophan, Fermentation, Chemistry, Chromatography, Absorption (acoustics), Standard curve, Food science, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination the assay of tryptophan in fermentation broth by Fe(AC)_3 — Research Paper | ScholarLens