A Method of Small-scale and Rapid Isolation of Total RNA from Wheat

The total RNA was isolated from young roots,stems and leaves of wheat seedlings by CTAB and urea method.The results showed that the total RNA isolated by CTAB method displayed clear bands of 28S rRNA and 18S rRNA on 1%(w/v) agarose gels;the value of A260/A280 approached 2;and RT-PCR analysis demonstrated that total RNA had high quality.Total RNA was successfully isolated from leaves and stems through urea method,but not from roots,indicating that CTAB method is superior to urea method.At the same time,the liquid nitrogen was not used in present study,thus save the cost of experiments.