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A Fast and Effective Isolation Method of Total RNA in Cereals

Tianyu Wang

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Abstract

The modified hot phenol method was used to extract total RNA from different tissues of maize,rice and wheat.The results showed that high quality RNA was obtained.The RNA isolated by the improved method showed clear bands of 28S rRNA and 18S rRNA,and the value of OD260/OD280 is 1.8 to 2.1.RNA isolated by the improved method could ba used for RT-PCR and Northern blot.

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What this paper is about

The modified hot phenol method was used to extract total RNA from different tissues of maize,rice and wheat.The results showed that high quality RNA was obtained.The RNA isolated by the improved method showed clear bands of 28S rRNA and 18S rRNA,and the value of OD260/OD280 is 1.8 to 2.1.RNA isolated by the improved method could ba used for RT-PCR and Northern blot.

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Available abstract

The modified hot phenol method was used to extract total RNA from different tissues of maize,rice and wheat.The results showed that high quality RNA was obtained.The RNA isolated by the improved method showed clear bands of 28S rRNA and 18S rRNA,and the value of OD260/OD280 is 1.8 to 2.1.RNA isolated by the improved method could ba used for RT-PCR and Northern blot.

Key concepts: RNA, 18S ribosomal RNA, Ribosomal RNA, 28S ribosomal RNA, RNA extraction, Molecular biology, Biology, Gene

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