Research on the Methods of Isolation of RNA from Oilseeds(Brassica napus. L)

Based on the methods of CTAB,SDS and Plant(Leaves) Total RNA Isolation Kit,total RNA was isolated from seeds of 40 days of flower from Braccica napus.L.The total RNA was compared by gel electrophoresis and the ratio of A260/A280 and A260/A230.RNA only was separated successfully from the seeds by the methods of CTAB.The sharpness of 28S bands was twice than 18S of bands and the ratio of A260/A280 and A260/A230 of RNA was 1.94 and 2.06,respectively.It indicated the quality of RNA was high,reverse transcription proved the quality of RNA was fine enough for ecumenical experimentation.