Effects of adrenomedullin 2 on proliferation of microvascular endothelial cells from the rat cerebral cortex

AIM: To investigate the effects of adrenomedullin(ADM) 2(AM_2) on proliferation of microvascular endothelial cells from the rat cerebral cortex.METHODS: Microvascular endothelial cells(MEC) were isolated from the cerebral cortex of SD rats and cultured.The cultured cells were identified using immunocytochemistry assay with antibody for factor VIII-related antigen and randomly distributed into eight experimental groups as follows: control,AM_2 10~(-7) mol/L,10~(-8) mol/L,10~(-9) mol/L,ADM,ADM+AM_2,10% fetal bovine serum(FBS) stimulated,and 10% FBS+AM_2 10~(-7) mol/L groups.The proliferation of MEC was detected using -TdR incorporation assay.RESULTS: Compared with control,AM_2(10~(-7)-10~(-9) mol/L),ADM(10~(-7) mol/L),and AM_2(10~(-7)mol/L) co-incubated with ADM(10~(-7) mol/L) had no effects on -TdR incorporation into the MEC(P0.05).10% FBS induced -TdR incorporation increased by 87.5%(vs control,P0.05),which was abolished by co-incubated the MEC with 10~(7) mol/L AM_2(P0.05).CONCLUSION: AM_2 inhibits FBS-stimulated proliferation of MEC from the rat cerebral cortex.