Induced apoptosis of renal tubular cells from neonate rats by angiotensinIIand AT_2 receptor

[Objective] To invest the role of angiotensinⅡand AT_2 receptor in apoptosis of renal tubular cells.[Methods] In vitro neonate Wistar rat's renal tubular cells culture,AngiotensinⅡ(AngⅡ) or AT_2 receptor agonist CGP-42112A in different doses were diffused separately,and at 24h,cells were analyzed by flow cytometry analysis of apoptosis,AT_2 receptor mRNA expression was measured by RT-PCR.[Results] In the groups with the diffusion of AngⅡ at 10~(-5)mol/L,10~(-6)mol/L,10~(-7)mol/L and 10~(-8)mol/L,apoptotic cell rations were(21.73±1.25)%,(18.65±0.49)%,(17.29±0.67)% and(15.98±0.71)% respectively,being higher than that in group(6.89±1.17)%,(P0.001).In the groups with diffusion at 10~(-5)mol/L,10~(-6)mol/L,10~(-7)mol/L and 10~(-8)mol/L of CGP-42112A,apoptotic cell rastions were that(22.32±2.69)%,(19.17±1.63)%,(17.98±1.96)% and((16.06±1.49)%) respectively,being higher than that of(7.04±0.61)%,(P0.001),a dose dependent manner was found,but difference was insignificant between AngⅡand CGP-42112A groups.RT-PCR results shown that the AT_2 receptor mRNA levels were up-regulated by both of CGP-42112A and AngⅡ.[Conclusions] AngiotensinⅡand CGP-42112A may up-regulated the renal tubular cells' apoptotic rations.The apoptosis was induced by AngiotensinⅡ maybe via the way of AT_2 receptor.