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Effects of Adrenomedullin1-50 on vascular cell adhesion molecule-1 expression in isolated rat heart

白育庭, 龙村, 涂仲凡, 关彬, 温福兴, 史世勇

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Abstract

目的探讨肾上腺髓质素 (Adm1-50)对缺血再灌注大鼠心肌组织血管细胞粘附分子-1(VCAM-1)表达的影响.方法 24只雄性SD大鼠,制作离体心脏缺血再灌注模型,并随机分为A、B、C、D 4组,每组6只.心脏缺血60 min,A组用氧合K-H液再灌注60 min,B、C、D组用氧合K-H液分别加入10-9mol/L、10-8mol/L、10-7mol/L的Adm1-50再灌注15 min,再用K-H液灌注45 min.逆转录-聚合酶链式反应(RT-PCR)法检测心肌组织VCAM-1 mRNA表达,并测定磷酸肌酸激酶同工酶(CK-MB)释放.结果 Adm1-50抑制再灌注大鼠心肌组织VCAM-1表达的作用呈浓度依赖性.Adm1-50减少了再灌注末心肌组织CK-MB漏出量,A组(31.5±3.3) U/L、B组(29.7±3.3) U/L、C组(24.3±3.0) U/L、D组(19.3±3.2) U/L,C、D组与A组比较P＜0.01.结论心肌缺血再灌注时,Adm1-50通过抑制VCAM-1 mRNA表达而产生心肌保护作用.

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目的探讨肾上腺髓质素 (Adm1-50)对缺血再灌注大鼠心肌组织血管细胞粘附分子-1(VCAM-1)表达的影响.方法 24只雄性SD大鼠,制作离体心脏缺血再灌注模型,并随机分为A、B、C、D 4组,每组6只.心脏缺血60 min,A组用氧合K-H液再灌注60 min,B、C、D组用氧合K-H液分别加入10-9mol/L、10-8mol/L、10-7mol/L的Adm1-50再灌注15 min,再用K-H液灌注45 min.逆转录-聚合酶链式反应(RT-PCR)法检测心肌组织VCAM-1 mRNA表达,并测定磷酸肌酸激酶同工酶(CK-MB)释放.结果 Adm1-50抑制再灌注大鼠心肌组织VCAM-1表达的作用呈浓度依赖性.Adm1-50减少了再灌注末心肌组织CK-MB漏出量,A组(31.5±3.3) U/L、B组(29.7±3.3) U/L、C组(24.3±3.0) U/L、D组(19.3±3.2) U/L,C、D组与A组比较P＜0.01.结论心肌缺血再灌注时,Adm1-50通过抑制VCAM-1 mRNA表达而产生心肌保护作用.

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Available abstract

目的探讨肾上腺髓质素 (Adm1-50)对缺血再灌注大鼠心肌组织血管细胞粘附分子-1(VCAM-1)表达的影响.方法 24只雄性SD大鼠,制作离体心脏缺血再灌注模型,并随机分为A、B、C、D 4组,每组6只.心脏缺血60 min,A组用氧合K-H液再灌注60 min,B、C、D组用氧合K-H液分别加入10-9mol/L、10-8mol/L、10-7mol/L的Adm1-50再灌注15 min,再用K-H液灌注45 min.逆转录-聚合酶链式反应(RT-PCR)法检测心肌组织VCAM-1 mRNA表达,并测定磷酸肌酸激酶同工酶(CK-MB)释放.结果 Adm1-50抑制再灌注大鼠心肌组织VCAM-1表达的作用呈浓度依赖性.Adm1-50减少了再灌注末心肌组织CK-MB漏出量,A组(31.5±3.3) U/L、B组(29.7±3.3) U/L、C组(24.3±3.0) U/L、D组(19.3±3.2) U/L,C、D组与A组比较P＜0.01.结论心肌缺血再灌注时,Adm1-50通过抑制VCAM-1 mRNA表达而产生心肌保护作用.

Key concepts: Cell adhesion molecule, Cell biology, Cell adhesion, Biology, Chemistry, Molecular biology, Cell, Genetics

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Effects of Adrenomedullin1-50 on vascular cell adhesion molecule-1 expression in isolated rat heart — Research Paper | ScholarLens