2011•China Animal Husbandry & Veterinary MedicineRequires access
Identificationand Biological Characterization of a Newcastle Disease Virus from Duck
Tao Ren
Open publisher page 0 citations
Abstract
A Newcastle disease virus(NDV) was isolated from duck in Guangdong.It was identified with hemagglutination,hemagglutination inhibition.These results showed the isolate had hemagglutination and was inhibited by the standard antibody of AIV-H5,AIV-H7,AIV-H9 and EDSV.It was amplified by RT-PCR and sequenced,which was used by the specific identification primers of NDV F gene.Thus,the isolate was identified as a duck NDV,named NDV/DUCK/SD/2006.In this research,the isolate were conducted virulence determination.Results showed that,the MDT,and IVPI of the isolate were 51.75 h,2.44 respectively,suggesting it was a velogenic strain.