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Identification and Molecular Characteristics of One Strain of Newcastle Disease Virus Isolated from Duck

Zhuoming Qin

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Abstract

One strain of Newcastle disease virus (NDV) isolated from duck in Shandong was proved virulent in terms of median embryo lethal dose (ELD50) of 107.85/0.1 mL,mean death time (MDT) of 48.5 h,intracerebral pathogenicity index (ICPI) of 1.80,and intravenous pathogenicity index (IVPI) of 2.36. The cleavage site of F protein of the virus was 112RRQKRF117,and was accorded with highly pathogenic strain. By analyzing genotype and homology of F gene of SDLP09 and other NDV strains, it showed that SDLP09 belonged to genotype Ⅶ,and had higher homology with virulent NDV from wild duck.

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What this paper is about

One strain of Newcastle disease virus (NDV) isolated from duck in Shandong was proved virulent in terms of median embryo lethal dose (ELD50) of 107.85/0.1 mL,mean death time (MDT) of 48.5 h,intracerebral pathogenicity index (ICPI) of 1.80,and intravenous pathogenicity index (IVPI) of 2.36. The cleavage site of F protein of the virus was 112RRQKRF117,and was accorded with highly pathogenic strain. By analyzing genotype and homology of F gene of SDLP09 and other NDV strains, it showed that SDLP09 belonged to genotype Ⅶ,and had higher homology with virulent NDV from wild duck.

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Available abstract

One strain of Newcastle disease virus (NDV) isolated from duck in Shandong was proved virulent in terms of median embryo lethal dose (ELD50) of 107.85/0.1 mL,mean death time (MDT) of 48.5 h,intracerebral pathogenicity index (ICPI) of 1.80,and intravenous pathogenicity index (IVPI) of 2.36. The cleavage site of F protein of the virus was 112RRQKRF117,and was accorded with highly pathogenic strain. By analyzing genotype and homology of F gene of SDLP09 and other NDV strains, it showed that SDLP09 belonged to genotype Ⅶ,and had higher homology with virulent NDV from wild duck.

Key concepts: Newcastle disease, Virulence, Genotype, Virology, Virus, Biology, Virus strain, Pathogenicity

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