Antitumor activity of Kanglaite combined with doxorubicin in vitro

Objective To study the antitumor activity in vitro of Kanglaite combined with doxorubicin and related mechanism.Methods Kanglaite and doxorubicin were applied on the OS-732 cells respectively or jointly and inhibition rate was measured by MTT assay;The celluar shape were observed with inverted phase contrast microscope and fluorescence microscope;The apoptotic rate was analyzed with flow cytometry(FCM).Results Kanglaite had the effect of up-regulating Fas expression and inducing apoptosis;the inhibition rate was stagnated with 1 g·L~(-1) Kanglaite,if combined with 1 mg·L~(-1) doxorubicin,the inhibition rate was increased obviously(P0.01).Changes of Celluar shape and apoptotic rate also showed apoptosis-inducing effect of joint application,which was much stronger than those of respective application.Conclusions Combination of Kanglaite and doxorubicin show a strong killing effect on OS-732 cells.