Effects of Kanglaite Injection combined with oxaliplatin on the proliferation and apoptosis of SW480 cells

Objective: To investigate the effects of Kanglaite Injection combined with oxaliplatin on the proliferation and apoptosis of SW480 cells. Methods: The cell counting kit-8 (CCK-8) assay was used to examine the growth inhibition rates of SW480 cells treated with various concentrations of Kanglaite Injection or oxaliplatin alone or in combination. The apoptosis and cell cycle were examined by flow cytometry (FCM). The expression level of cell apoptosis-associated protein Bcl-2 was examined by immunocytochemistry and Western blotting. Results: The proliferation of SW480 cells was inhibited by Kanglaite Injection or oxaliplatin, and the synergistic effect of proliferation inhibition was observed when Kanglaite Injection and oxaliplatin were administered together (P ＜0.05). The cell cycle was blocked at G2/M phase (P ＜0.05). The apoptosis rate of SW480 cells treated with Kanglaite Injection combined with oxaliplatin was significantly higher than those treated with Kanglaite Injection or oxaliplatin alone [(23.30±3.04)%, (7.50±0.85)% and (9.00±2.14)%,respectively; P ＜0.05]. The immunocytochemistry and Western blotting methods revealed that the Kanglaite Injection could significantly enhance the inhibitory efficacy of Bcl-2 expression in SW480 cells treated with oxaliplatin (P ＜0.05). Conclusion: The cell proliferation inhibition and the apoptosis of SW480 cells can be synergistically enhanced by the treatment of Kanglaite Injection combined with oxaliplatin. DOI:10.3781/j.issn.1000-7431.2011.04.008