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Study on Isolation and Purification of Chlorogenic Acid in Flos Lonicerae

Hao Feng-xia

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Abstract

Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216 ≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry.

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What this paper is about

Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216 ≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry.

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Available abstract

Objecive: To study the isolation and purification conditions of chlorogenic acid(CA) from Flos Lonicerae. Method: The isolation and purification of CA were achieved by polyamide column chromatography and the main isolation factors were optimized. Results: The optimum isolation is as following: the rate of crude CA to polymide is 1:5, the rate of diameter to long is 1:7, 10% alcohol washed at high speed under usual pressure, CA is collected between the volume of 5 ～ 14 times of column volume(A322/A216 ≥0.9), under the conditions, the product with above 70% CA is obtained, which can be further crystalled by EtoAc and the purity is enhanced to 93%. Conclusion: This method is simple, low cost, and suitable to be used in industry.

Key concepts: Flos, Chlorogenic acid, Polyamide, Chromatography, Chemistry, Recovery rate, Volume (thermodynamics), Isolation (microbiology)

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