Simultaneous Extraction and Isolation of Chlorogenic Acid and Flavonoids from Flos lonicerae

Chlorogenic acid and flavonoids were simultaneously extracted by ethanol with the aid of ultrasonic treatment from Flos lonicerae and then isolated by polyamide column chromatography.Results showed that three products(A, B and C) were obtained from column chromatography with gradient elution, which were identified to be chlorogenic acid, soluble flavonoid and lipid-soluble flavonoid, respectively.The chlorogenic acid content in product A was 68.2% with a yield of 1.34%;the flavonoid content in product B was 95.0% with a yield of 1.12%;and the quercetin and lonicertin contents in product C were 7.5 and 16.9%, respectively, with a yield of 0.11%.