Extraction and the Dynamic Content of Total Flavonoids in Patrina

Patrina was used as the raw material to extract total flavonoids;and the dynamic change of flavonoids in patrina during growth was examined.To optimize the extraction techniques of total flavonoids,technological parameters including extraction time,solid to liquid ratio,temperature and ethanol concentration were investigated by single factor method coupling with L9(34) orthogonal array design.Meanwhile,the effect of ultrasonic was also studied.Results showed that the maximum extraction ratio of total flavonoids was obtained by using 50% ethanol with a solid to liquid ratio of 1∶20 and extracting for 2.0 h at 60℃.The content of total flavonoids was increased by using ultrasonic technique,and was the highest in tender levels of patrina.