Time-dose-effect Studies on Hepatotoxicity of Mice Induced by Fructus Toosendan

Objective To study the time-effect relation and dose-effect relation of hepatotoxicity induced by Fructus Toosendan in mice.Methods The medial lethal dose(LD50) of mice was calculated after different doses of Fructus Toosendan was given orally.Then the mice were divided into different groups according to different doses or time,and the contents of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)were investigated,the thymus,liver and spleen were weighed,and the liver tissue was studied with histopathological to observe the degrees of inflammatory activity and fibrous hyperplasia.Results The LD50 of Fructus Toosendan was 200 g/kg,95% confidence level of which was 192～212 g/kg,ALT,AST in serum reached its peak after 1 hour,which recovered closely to normal value after 24 hours.Compared with control group,hepatic tissues of the high and medium dose groups were damaged significantly,and the contents of ALT and AST in serum were increased significantly(P0.05 or P0.01).Conclusion When the mice were fed with a certain dose of Fructus Toosendan relation for single time,it will be induced acute liver toxicity and even death.Time-dose-toxicity effects would be indicated of Fructus Toosendan.