Protective effect of lignanamides extract from Fructus Cannabis of Bama on acute hepatic injury in mice

Objective To observe the protective effect of lignanamides extract from Fructus Cannabis(LFC) on acute hepatic injury induced by carbon tetrachloride in mice. Methods KM mice were pretreated with LFC low dose(60 mg·kg- 1), middle dose(120 mg·kg- 1), and high dose(180 mg·kg- 1) for 7 days. Mouse models of acute liver injury were established by the treatment of CCl4. After 16 hours, the activities of AST and ALT in the serum, MDA level and the activity of SOD in the liver were measured by the detection kits according to the manufacturer protocols. The liver histopathological characters were observed under the microscope. Results At each dose of LFC, the activities of AST and ALT in the serum(P 0.01) and MDA level in the liver(P 0.01) were significantly reduced, the activity of SOD in the liver was significantly increased compared with that in the CCl4 model group. Under the microscope, liver injury induced by CCl4 was greatly decreased in each LFC dose group. Conclusion LFC has protective effect on liver damage induced by CCl4 in mice.