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2010•Yunnan yiyaoRequires access

Expression of MMP-9 Gene after Focal Cerebral Ischemia and Reperfusion in rat

Ji Liu

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Abstract

Objective To explore the expression of MMP-9 gene in different times after focal cerebral ischemia and reperfusion in the rat.Methods The middle cerebral artery occlusion 90 min and reperfusion 12 h、24 h、48h and 7d models were built in rat.The expressions of MMP-9 gene were observed by Western blotting and Real-time PCR.Result The expressions of MMP-9 protein and mRNA increased remarkably after ischemia and reperfusion 12 h、24 h、48h and 7d.It had a peak at 48h,and started to decrease at 7 d.Conclusion The expressions of MMP-9 gene increase in the focal cerebral ischemia and reperfusion in the rat,this indicates MMP-9 plays an important role in focal cerebral ischemia and reperfusion.

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What this paper is about

Objective To explore the expression of MMP-9 gene in different times after focal cerebral ischemia and reperfusion in the rat.Methods The middle cerebral artery occlusion 90 min and reperfusion 12 h、24 h、48h and 7d models were built in rat.The expressions of MMP-9 gene were observed by Western blotting and Real-time PCR.Result The expressions of MMP-9 protein and mRNA increased remarkably after ischemia and reperfusion 12 h、24 h、48h and 7d.It had a peak at 48h,and started to decrease at 7 d.Conclusion The expressions of MMP-9 gene increase in the focal cerebral ischemia and reperfusion in the rat,this indicates MMP-9 plays an important role in focal cerebral ischemia and reperfusion.

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Available abstract

Objective To explore the expression of MMP-9 gene in different times after focal cerebral ischemia and reperfusion in the rat.Methods The middle cerebral artery occlusion 90 min and reperfusion 12 h、24 h、48h and 7d models were built in rat.The expressions of MMP-9 gene were observed by Western blotting and Real-time PCR.Result The expressions of MMP-9 protein and mRNA increased remarkably after ischemia and reperfusion 12 h、24 h、48h and 7d.It had a peak at 48h,and started to decrease at 7 d.Conclusion The expressions of MMP-9 gene increase in the focal cerebral ischemia and reperfusion in the rat,this indicates MMP-9 plays an important role in focal cerebral ischemia and reperfusion.

Key concepts: Ischemia, Medicine, Matrix metalloproteinase, Blot, Messenger RNA, Middle cerebral artery, Gene expression, Gene

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