Construction of a recombinant adenovirus carrying human BMP2 gene and its function of inducing bone formation

Objective To construct a recombinant adenovirus carrying human BMP2 gene,and study its function of inducing bone formation.Methods BMP2 gene was cloned into the shuttle vector-pADtrack;the bacterium BJ5183 was transfected with the shuttle and the pADEasy vector and the recombinant adenoviral plasmid was produced by homologous recombination.Recombinant adenovirus was packaged in HEK293 cells.Then bone formation was studied in nude mice.Results PCR and endonucleas digesting demonstrated that the shuttle plasmid-pADTrack-BMP2 and the recombinant adenoviral plasmid were successfully obtained.The recombinant adenovirus was packaged in HEK293 cells.Histological method showed new bone formation at 5W.Conclusion Construction of BMP2 recombinant adenovirus and its induction of new bone formation was the basis of BMP2 gene therapy.