Construction of human BMP7 recombinant adenovirus and its function of inducing bone formation

Objective To construct and express a recombinant adenovirus carrying human BMP7 gene, and study its function of inducing bone formation. Methods BMP7 gene was cloned into the shuttle vector-pADTrack, and cotransfected into bacterium BJ5183 together with the pADEasy vector to produce recombi-nant adenoviral plasmid by homologous recombination. Recombinant adenovirus was packaged in HEK293 cells. Then bone formation was studied in nude mice. Results PCR and digesting demonstrated that the shuttle plasmid-pADTrack-BMP7 and the recombinant adenoviral plasmid were obtained. The recombinant adenovi-rus was packaged in HEK293 cells. X-ray and histological methods showed new bone formation at 5W. Conclusions Construction and expression of human BMP7 recombinant adenovirus and its induction of new bone formation lay the basis for BMP7 gene therapy.