Construction and expression of human BMP7 reconbinant adenovirus and Transfection into marrow mesenchymal stem cells

Objective To construct a recombinant adenovirus carrying human BMP7 gene which is transfected into marrow mesenchymal stem cells (MSCs) and expressed.Methods BMP7 gene was cloned into the shuttle vector pADTrack; the bacterium BJ5183 was contransfected with the shuttle and the pADEasy vector and the recombinant adenoviral plasmid was produced by homologous.Recombinant adenovirus was packaged in HEK293 cells.Marrow MSCs were transfected with recombinant adenovirus and BMP7 expression was confirmed.Results PCR and digesting demonstrated that the shuttle plasmid pADTrack BMP7 and the recombinant adenoviral plasmid were obtained.The recombinant adenovirus was packaged in HEK293 cells.RT PCR and immunohistochemical methods revealed that BMP7 was expressed in MSCs.Conclusion Construction of human BMP7 recombinant adenovirus and its expression in marrow MSCs laid the basis for BMP7 gene therapy.