Effect of sodium butyrate on configurition and growth curve of HT-29 cells.

Objective Observing the change of configurition and growth curve of HT-29 cells treated with sodium butyrate and the effect of sodium butyrate on induction of apoptosis in colonic neoplasm cells. Methods Cells were observed by PCM, BVPM, EM .MTT examine the growth of HT-29 cells. Results Configurition of HT-29 cells were changed after treatment with sodium butyrate. The cells became smaller, shape of cells rounder and pyknosis, shedding cells increased gradually. The number of protrusion of cell membrane decreased. Chromatin accumulate under the nuclear envelope . Apoptosis bodies appeared also by EM. 2.5、5 and 10mM sodium butyrate all can cause growth-inhibition. The effects were time-and dose-dependent.Conclusion Sodium butyrate have an effect on induction of apoptosis in colonic neoplasm cells.