ScholarLens Back to search
2004•DOAJ (DOAJ: Directory of Open Access Journals)Requires access

Production and characterization of monoclonal antibody to recombinant chicken IL-2

LI Zeng-kui

Open publisher page 1 citations

Abstract

Four hybridoma cell lines named as 1A12, 4A3, 3E7 and 4E1, secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows: The ELISA titers were 5.12× 105, 1.02×106, 2.56×105 and 4.09×106, respectively. All the Mabs failed into type IgG1. Western-blot analysis showed that the four MAbs could react with chicken IL-2.

About this research paper

What this paper is about

Four hybridoma cell lines named as 1A12, 4A3, 3E7 and 4E1, secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows: The ELISA titers were 5.12× 105, 1.02×106, 2.56×105 and 4.09×106, respectively. All the Mabs failed into type IgG1. Western-blot analysis showed that the four MAbs could react with chicken IL-2.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Four hybridoma cell lines named as 1A12, 4A3, 3E7 and 4E1, secreting monoclonal antibody (MAb) against chicken IL-2 were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/c immunized with the purified recombinant chicken IL-2 protein. The four MAbs were identified as follows: The ELISA titers were 5.12× 105, 1.02×106, 2.56×105 and 4.09×106, respectively. All the Mabs failed into type IgG1. Western-blot analysis showed that the four MAbs could react with chicken IL-2.

Key concepts: Monoclonal antibody, Recombinant DNA, Molecular biology, Titer, Biology, Western blot, Antibody, Spleen

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Production and characterization of monoclonal antibody to recombinant chicken IL-2 — Research Paper | ScholarLens