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2013•Zhongguo yufang shouyi xuebaoRequires access

Development and characterization of monoclonal antibodies against chicken β_2-microglobulin

Aijian Qin

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Abstract

Two monoclonal antibodies(MAbs) against chicken β2-microglobulin(β2-M) were prepared by fusions the SP2/0 cells with spleen cells from BALB/c mice immunized with a synthesized peptide(TF-29).The ELISA titers of two antibodies 3D1 and 6E7,identified as IgG1/κ,were 1∶25 600 and 1∶204 800 respectively.Both MAbs specifically recognized not only the recombinant β2-M expressed in prokaryotic cells,but also the native chicken β2-M in avian macrophage HD11 cells by western blot and immunofluorescence assays.Moreover,both MAbs were able to react with the β2-M from chicken serum and plasma in western blot assay.These MAbs would facilitate for further study of chicken β2-M in the regulation of the immune system in chicken.

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What this paper is about

Two monoclonal antibodies(MAbs) against chicken β2-microglobulin(β2-M) were prepared by fusions the SP2/0 cells with spleen cells from BALB/c mice immunized with a synthesized peptide(TF-29).The ELISA titers of two antibodies 3D1 and 6E7,identified as IgG1/κ,were 1∶25 600 and 1∶204 800 respectively.Both MAbs specifically recognized not only the recombinant β2-M expressed in prokaryotic cells,but also the native chicken β2-M in avian macrophage HD11 cells by western blot and immunofluorescence assays.Moreover,both MAbs were able to react with the β2-M from chicken serum and plasma in western blot assay.These MAbs would facilitate for further study of chicken β2-M in the regulation of the immune system in chicken.

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Available abstract

Two monoclonal antibodies(MAbs) against chicken β2-microglobulin(β2-M) were prepared by fusions the SP2/0 cells with spleen cells from BALB/c mice immunized with a synthesized peptide(TF-29).The ELISA titers of two antibodies 3D1 and 6E7,identified as IgG1/κ,were 1∶25 600 and 1∶204 800 respectively.Both MAbs specifically recognized not only the recombinant β2-M expressed in prokaryotic cells,but also the native chicken β2-M in avian macrophage HD11 cells by western blot and immunofluorescence assays.Moreover,both MAbs were able to react with the β2-M from chicken serum and plasma in western blot assay.These MAbs would facilitate for further study of chicken β2-M in the regulation of the immune system in chicken.

Key concepts: Monoclonal antibody, Biology, Western blot, Antibody, Molecular biology, Immunofluorescence, Spleen, Recombinant DNA

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