ScholarLens Back to search
2008•Zhongguo yufang shouyi xuebaoRequires access

Development of monoclonal antibodies against chicken IL-2

Jiao Xin-an

Open publisher page 0 citations

Abstract

Sp2/0-Ag-14 myeloma cells was fused with splenocytes from BALB/c mice immunized against chicken IL-2 and the supernatant of hybridoma clones were screened by indirect ELISA. Two hybridoma cell lines 1H10 and 1E6 secreting moloc- lonal antibodies (Mab) against ChIL-2 were obtained. The mAbs had an ascites titers pf 1:6 400 and 1:3 200 respectively and both were subtype IgM. IFA assay revealed that both mAbs could react with COS-7 cells transfected by pcDNA3.1-ChIL-2. Western blot analysis confirmed that these mAbs specifically recognized ChIL-2 only. Therefore the McAb developed in this work could be a useful tool to study avian immune cell function and immune regulations, and used as an effective reagent for the detection of chicken IL-2.

About this research paper

What this paper is about

Sp2/0-Ag-14 myeloma cells was fused with splenocytes from BALB/c mice immunized against chicken IL-2 and the supernatant of hybridoma clones were screened by indirect ELISA. Two hybridoma cell lines 1H10 and 1E6 secreting moloc- lonal antibodies (Mab) against ChIL-2 were obtained. The mAbs had an ascites titers pf 1:6 400 and 1:3 200 respectively and both were subtype IgM. IFA assay revealed that both mAbs could react with COS-7 cells transfected by pcDNA3.1-ChIL-2. Western blot analysis confirmed that these mAbs specifically recognized ChIL-2 only. Therefore the McAb developed in this work could be a useful tool to study avian immune cell function and immune regulations, and used as an effective reagent for the detection of chicken IL-2.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Sp2/0-Ag-14 myeloma cells was fused with splenocytes from BALB/c mice immunized against chicken IL-2 and the supernatant of hybridoma clones were screened by indirect ELISA. Two hybridoma cell lines 1H10 and 1E6 secreting moloc- lonal antibodies (Mab) against ChIL-2 were obtained. The mAbs had an ascites titers pf 1:6 400 and 1:3 200 respectively and both were subtype IgM. IFA assay revealed that both mAbs could react with COS-7 cells transfected by pcDNA3.1-ChIL-2. Western blot analysis confirmed that these mAbs specifically recognized ChIL-2 only. Therefore the McAb developed in this work could be a useful tool to study avian immune cell function and immune regulations, and used as an effective reagent for the detection of chicken IL-2.

Key concepts: Monoclonal antibody, Splenocyte, Biology, Molecular biology, Immune system, Titer, Antibody, Virology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Development of monoclonal antibodies against chicken IL-2 — Research Paper | ScholarLens