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Conditions optimization of peanut protein isolate productionby alkaline extraction and acid precipitation

LI Ming-shu

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Abstract

Alkaline extraction and acid precipitation were employed to produce peanut protein isolate.The results of single-factor and orthogonal experiments showed the optimum conditions of isolation.After defatted peanut flour was mixed with distilled water in the ratio of 1∶8(m/V) and the pH was adjusted to 8.2,the suspension was extracted at 60℃ for 2 h.Repeating the process above,peanut protein was precipitated to its maximum in the condition of pH4.5.The purity of the isolated protein can reach 90.21% and the utilization ratio is 75.74%.

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What this paper is about

Alkaline extraction and acid precipitation were employed to produce peanut protein isolate.The results of single-factor and orthogonal experiments showed the optimum conditions of isolation.After defatted peanut flour was mixed with distilled water in the ratio of 1∶8(m/V) and the pH was adjusted to 8.2,the suspension was extracted at 60℃ for 2 h.Repeating the process above,peanut protein was precipitated to its maximum in the condition of pH4.5.The purity of the isolated protein can reach 90.21% and the utilization ratio is 75.74%.

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Available abstract

Alkaline extraction and acid precipitation were employed to produce peanut protein isolate.The results of single-factor and orthogonal experiments showed the optimum conditions of isolation.After defatted peanut flour was mixed with distilled water in the ratio of 1∶8(m/V) and the pH was adjusted to 8.2,the suspension was extracted at 60℃ for 2 h.Repeating the process above,peanut protein was precipitated to its maximum in the condition of pH4.5.The purity of the isolated protein can reach 90.21% and the utilization ratio is 75.74%.

Key concepts: Distilled water, Protein isolate, Extraction (chemistry), Chemistry, Chromatography, Protein purification, Precipitation, Water extraction

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