Induction of Bulblets from Shoot Apex in Vitro and Cultivation of Flower Bulb in Asiatic Hybrid Lily

In this experiment bud induction from shoot apex, bulblet induction from buds in vitro culture and flower bulb cultivation from bulblet in field were carried out in Asiatic hybrid lily. The results showed that bud induction rate was as high as 100% on the MS medium containing BA 0.5 mg/L and NAA 0.1 - 0.2 mg/L. The small bud proliferated constantly and became bud seedlings, and their proliferation coefficient was six on the MS medium with BA 0.5 mg Land NAA 0.1 - 0.2 mg/L. Bulblets could be induced by bud seedlings in test tubes. Many factors like plant hormone, sugar, illumination intensity and low temperature affected bulblet formation. Under the condition of illumination intensity for 1.5 klx, on the MS added with IBA 0.5 mg/L + sugar 4% , bud seedlings have a better effect on inducing growing bulblets, with 100% induction rate and 0.96 cm diameter of inducing bulblet, and 1.5 average bulblets in one bud seedling. Under the condition of low temperature 5℃ for 2 weeks, the test-tube bulblet regeneration occurred on the MS medium supplemented with BA 0.1 mg/L + NAA 0.5 mg/L, their diving rate reaches 82.5% ,4.2 little bulbs for each bud scale and 0.29 cm diameter of little bulb. By treating with low temperature 5 ~ 8℃ for 4 weeks, the test-tube bulbs, the diameter of which was over 0.8 cm, were cultivated for eight months at a mountain of the elevation of 800 m near Nanping in Fujian. 45.8 % of the new bulbs harvested were big enough and up to the standard of commercial bulbs, available to produce cut flowers.Their ornamental characters were stable.