2008•Journal of Fruit and Ornamental Plant ResearchRequires access
BULBLET REGENERATION FROM IN VITRO ROOTS OF ORIENTAL LILY HYBRID
Surinder Kumar, Vinita Chaudhary, J. K. Kanwar
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Abstract
Root segments from the middle and distal zones of in vitro roots of oriental lily hybrid cultivar 'Rosato' were isolated and cultured on MS nutrient medium supplemented with IBA, NAA and 2,4-D alone or in combination with BA. Hormone-free MS medium was ineffective in inducing bulblet regeneration. Bulblets were induced when the medium contained either 1.0, 1.5 and 2.0 mg 1 -1 NAA, 2.0 mg 1 -1 2.4-D or 1.5 and 2.0 mg 1 -1 BA. A significant increase in the percentage of explants producing bulblets and the number of bulblets per an explant was observed on medium containing 2 mg 1 -1 NAA in combination with 2 mg 1 -1 BA. After ninety days of culture, the weighty bulblets were recorded on medium supplemented with 1 mg 1 -1 NAA and 1.5 mg 1 -1 BA. The bulblets attaining the size of 14-16 cm flowered in two years in pots without any phenotypic variations.