2010•Unpublished venueRequires access
In vitro culture of bulblets regeneration derived from bulb scales of tulip
Fang Feng
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Abstract
The bulb scales of two tulip cultivars,viz.,Little angel and Christmas were used to study the effects of different inoculation methods,different parts of bulb scale as explant,genotypes and hormone concentrations on in vitro regeneration of bulblet derived from different parts of bulb scale.The survival rate of bulb scales was recorded higher(76.67%) when the convexity of bulb scale was upward.The survival rate of inner bulb scale reached 85.00%.The bulb scale of variety Little angle had the highest survival rate and bulblet differentiation rate,when cultured on MS medium supplemented with 6-BA(1.0 mg/L) and NAA(0.5 mg/L),while variety Christmas had higher bulblet differentiation rate on MS medium supplemented with 6-BA(2.0 mg/L) and NAA(0.5 mg/L).The induced bulblet of two varieties showed best proliferation on MS medium supplemented with 6-BA(3.0 mg/L) and NAA(0.2 mg/L).