Experimental study of effect of invigorating kidney for consolidating semen Chinese medicine on expression of Smurf1 signal transduction protein in the femur of osteoporotic rats

Objective To explore the pathogenesis of osteoporosis by observation of the alteration of Smurf1 expression in rat femurs of ovariectomized osteoporosis model.Methods The osteoporosis rat model was established using ovriectomy method.The Nano-calcium Chinese medicine that has invigorating kidney for consolidation semen function(high and low dose) was administered to modeled rats for 12 weeks.Gushukang granule and Gaitianli tablets groups were as positive control.Normal control group,sham operation group,and model control group were used.Buzhong yiqi decoction to invigorating spleen group was for comparison.The expression of Smurf1 proteins was measured using IHC method.Results Smurf1 protein positive expression in rats of model group decreased significantly,compared to that of normal control group(P 0.01).Smurf1 protein expression in all dose groups increased,compared to that of the model control group after a 12-week drug use(P 0.01).Conclusion The decreasing of Smurf1 protein expression in the bone tissue may be one of the mechanisms of osteoporosis pathogenesis.The invigorating kidney for consolidation semen Chinese medicine may prevent and treat osteoporosis by improving and regulating the expression of Smurf1 protein.