Delete studies on expressions of Smurf1 signal transduction proteins in the femur,kidney,hypothalamus tissue in oophorectomize inducted osteoporosis rat

Objective:To approach the molecular biological mechanism of kidney deficiency inducing osteoporosis by chasing the change of Smurf1 in location and activity in femur,kidney,hypothalamus.Methods:Oophorectomized female rat was taken as the osteoporosis rat model;treated the osteoporosis rat with experimental drugs low-dosage and high dosage of reinforcing kidney herb group for 12 weeks.Simultaneously,delete Gushukang Granule and Gaitianli Tablets and Buzhong Yiqi Decoction were taken as positive control groups.Normal control,sham control and model control were set for comparing.We used the IHC method to investigate the expression of Smurf1 proteins in the hypothalamus,kidney and femurof the rats.Results:The Smurf1 protein expression in femur:model control group got a decreasd in Smurf1 protein expression compared with normal groups(P0.01).Different level increase were detected in all medicine groups,among which nanometer calcium kidney-tonifying herbs groups showed the most significantly change,compared with model control group.The Smurf1 protein expression in kidney:model groups got a obviously decreasd in Smurf1 protein expression compared with normal groups(P0.01).All medicine groups increasd Smurf1 protein expression to differrent level compared with model groups(P0.01).The Smurf1 protein expression in the hypothalamus of rats:model groups got a obviously increasd in Smurf1 protein expression compared with normal groups(P0.01).All medicine groups decreasd Smurf1 protein expression to differrent level compared with model groups(P0.01).Conclusion:The decreasing of Smurf1 protein expression in the kidney and femur of rats while the increasing of Smurf1 protein expression in hypothalamus may be the one of the mechanism of osteoporosis due to kidney deficiency.The Chinese herbs with kidney-tonifying,calcium-supplying action are able to prevent and cure osteoporosis due to kidney deficiency indirectly by regulating the expression of Smurf1.