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2012•Chinese Journal of Analysis LaboratoryRequires access

Determination of Bombyx mori tyrosine hydroxylase activity by high performance liquid chromatography with UV detection

XU Wei-an

Open publisher page 1 citations

Abstract

A method for the rapid determination of bombyx mori tyrosine hydroxylase activity by high performance liquid chromatography with UV detection was proposed.The analytical column was a Venusil XBP C18 column(4.6mm×100mm,5μm),and the mobile phase was adjusted to pH3.5 with hydrochloric acid,the flow rate was set 0.5mL/min,UV detection was set at 280 nm,and column temperature was 30℃.The linear concentration range was 0.1~100μg/mL with good correlation coefficient of 0.9991(n=6).The detection limit was 0.02μg/mL(S/N≥3).The RSDs of intraday and interday were between 3.1% and 12% of middle and high concentration samples.The sample recoveries were between 89.0% and 104.6%(n=6).The method is rapid,stable and highly sensitive.It is suitable for the determination of insect tyrosine hydroxylase activity.

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What this paper is about

A method for the rapid determination of bombyx mori tyrosine hydroxylase activity by high performance liquid chromatography with UV detection was proposed.The analytical column was a Venusil XBP C18 column(4.6mm×100mm,5μm),and the mobile phase was adjusted to pH3.5 with hydrochloric acid,the flow rate was set 0.5mL/min,UV detection was set at 280 nm,and column temperature was 30℃.The linear concentration range was 0.1~100μg/mL with good correlation coefficient of 0.9991(n=6).The detection limit was 0.02μg/mL(S/N≥3).The RSDs of intraday and interday were between 3.1% and 12% of middle and high concentration samples.The sample recoveries were between 89.0% and 104.6%(n=6).The method is rapid,stable and highly sensitive.It is suitable for the determination of insect tyrosine hydroxylase activity.

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Available abstract

A method for the rapid determination of bombyx mori tyrosine hydroxylase activity by high performance liquid chromatography with UV detection was proposed.The analytical column was a Venusil XBP C18 column(4.6mm×100mm,5μm),and the mobile phase was adjusted to pH3.5 with hydrochloric acid,the flow rate was set 0.5mL/min,UV detection was set at 280 nm,and column temperature was 30℃.The linear concentration range was 0.1~100μg/mL with good correlation coefficient of 0.9991(n=6).The detection limit was 0.02μg/mL(S/N≥3).The RSDs of intraday and interday were between 3.1% and 12% of middle and high concentration samples.The sample recoveries were between 89.0% and 104.6%(n=6).The method is rapid,stable and highly sensitive.It is suitable for the determination of insect tyrosine hydroxylase activity.

Key concepts: Chemistry, Chromatography, Detection limit, Tyrosine hydroxylase, Linear range, High-performance liquid chromatography, Correlation coefficient, Bombyx mori

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